{"created":"2023-05-15T12:31:32.674560+00:00","id":573,"links":{},"metadata":{"_buckets":{"deposit":"e260c76c-6656-4975-8e75-591d637b2b67"},"_deposit":{"created_by":7,"id":"573","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"573"},"status":"published"},"_oai":{"id":"oai:hirogaku-u.repo.nii.ac.jp:00000573","sets":["43:44:102"]},"author_link":["509","516","1657","502","1656","1663","1664","1653","1661","1654","1659","1660","1655","1662","1658"],"item_2_alternative_title_21":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Experimental study for elucidating mechanism of coronary spasm using iPSC; role of p122 RhoGAP / DLC-1 and phospholipase C"}]},"item_2_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"51","bibliographicPageStart":"45","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"弘前学院大学看護紀要 "}]}]},"item_2_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【背景と目的】我々は冠攣縮性狭心症患者（CSA）の皮膚線維芽細胞の Phospholipase C（ PLC）活性が亢進していることを報告してきた。本研究は，冠攣縮性狭心症のメカニズムを解明するために，CSA 患者および対照被験者の皮膚線維芽細胞から人工多能性幹細胞（iPSC）を樹立し，更に，iPSCを平滑筋細胞（SMC）に分化誘導させ，CSA の病態に関与する細胞内Ca2+ 流入の分子機構を調べた。\n【方法と結果】細胞内遊離Ca2+ 濃度（［Ca2+］i）はFura-2 AM を使用し測定した。アセチルコリン（ACh）10-4M 誘導性の［Ca2+］i は，対照iPSC 由来SMC と比しCSA iPSC 由来SMC で優位に上昇した（53±10 versus 129±24 nM, p <0.05）。前処理ジルチアゼム10-4M では，対照iPSC 由来SMC では42±1nM（p <0.05），CSA iPSC 由来SMC では72±10 nM（p <0.01）に抑制された。ウェスタンブロット解析の結果，TRPC3 およびPLC-δ1 は差はなかったが，p122RhoGAP/DLC-1 およびTRPC6 の蛋白発現は，対照iPSC 由来SMC と比しCSA iPSC 由来SMC で，それぞれ2.60±0.8および1.68±0.28倍増加していた（p ＜0.05）。PLC 活性は，対照iPSC 由来SMC に比しCSA iPSC 由来SMC において有意に高かった（3.12±1.28倍，p <0.05）。\n【結論】DLC-1 およびTRPC6 タンパク質の過剰発現とPLC 活性の増強は，CSA 患者における細胞内［Ca2+］i 濃度の増加に寄与している。","subitem_description_type":"Abstract"}]},"item_2_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"1657","nameIdentifierScheme":"WEKO"}],"names":[{"name":"タナカ, マコト"}]},{"nameIdentifiers":[{"nameIdentifier":"1658","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オサナイ, トモヒロ"}]},{"nameIdentifiers":[{"nameIdentifier":"1659","nameIdentifierScheme":"WEKO"}],"names":[{"name":"オクムラ, ケン"}]},{"nameIdentifiers":[{"nameIdentifier":"1660","nameIdentifierScheme":"WEKO"}],"names":[{"name":"トミタ, ヒロフミ"}]},{"nameIdentifiers":[{"nameIdentifier":"509","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ヨシオカ, トシタダ"}]}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"1661","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tanaka, Makoto"}]},{"nameIdentifiers":[{"nameIdentifier":"1662","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Osanai, Tomohiro"}]},{"nameIdentifiers":[{"nameIdentifier":"1663","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Okumura, Ken"}]},{"nameIdentifiers":[{"nameIdentifier":"1664","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tomita, Hirofumi"}]},{"nameIdentifiers":[{"nameIdentifier":"516","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yoshioka, Toshitada"}]}]},"item_2_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"弘前学院大学看護学部"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12149754","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-8867","subitem_source_identifier_type":"ISSN"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"弘前学院大学看護学部看護学科"},{"subitem_text_value":"弘前大学大学院保健学研究科"},{"subitem_text_value":"済生会熊本病院循環器内科"},{"subitem_text_value":"弘前大学医学部循環器腎臓内科学講座"},{"subitem_text_value":"弘前学院大学看護学部看護学科"}]},"item_2_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田中, 真実"}],"nameIdentifiers":[{"nameIdentifier":"1653","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"長内, 智宏"}],"nameIdentifiers":[{"nameIdentifier":"1654","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"奥村, 謙"}],"nameIdentifiers":[{"nameIdentifier":"1655","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"富田, 泰史"}],"nameIdentifiers":[{"nameIdentifier":"1656","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉岡, 利忠"}],"nameIdentifiers":[{"nameIdentifier":"502","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","displaytype":"detail","filename":"HirogakuKangoKiyo_16_45.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://hirogaku-u.repo.nii.ac.jp/record/573/files/HirogakuKangoKiyo_16_45.pdf"},"version_id":"7df5ee42-9251-463a-bbec-40889ea10658"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"冠攣縮性狭心症","subitem_subject_scheme":"Other"},{"subitem_subject":"人工多能性幹細胞（iPSC）","subitem_subject_scheme":"Other"},{"subitem_subject":"Phospholipase C","subitem_subject_scheme":"Other"},{"subitem_subject":"アセチルコリン","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"冠攣縮性狭心症患者由来iPS 細胞を用いた病態の解明; p122RhoGAP/DLC-1 とphospholipase C の役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"冠攣縮性狭心症患者由来iPS 細胞を用いた病態の解明; p122RhoGAP/DLC-1 とphospholipase C の役割"}]},"item_type_id":"2","owner":"7","path":["102"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-12-03"},"publish_date":"2021-12-03","publish_status":"0","recid":"573","relation_version_is_last":true,"title":["冠攣縮性狭心症患者由来iPS 細胞を用いた病態の解明; p122RhoGAP/DLC-1 とphospholipase C の役割"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2023-05-15T13:07:44.005265+00:00"}